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BD Stemflow 人 MSC 分析試劑盒 562245

人多能間充質(zhì)基質(zhì)細胞分析試劑盒,BD Stemflow™ Human MSC Analysis Kit

描述：

細胞特性與鑒定標準

人源多能間充質(zhì)基質(zhì)細胞(Mesenchymal Stromal Cells, MSCs)，亦稱間充質(zhì)干細胞，是一類具有以下特征的成體干細胞：

• 來源多樣性：可從骨髓、脂肪等多種組織中分離獲得

• 多向分化潛能：經(jīng)體外培養(yǎng)可分化為脂肪細胞(adipocytes)、骨細胞(osteocytes)和軟骨細胞(chondrocytes)

• 免疫調(diào)節(jié)功能：在體內(nèi)外均表現(xiàn)出顯著的免疫調(diào)控活性

根據(jù)國際細胞治療協(xié)會(ISCT)2006年制定的標準，骨髓來源MSCs應滿足：

陽性標志物：CD73(+) | CD90(+) | CD105(+)
陰性標志物：CD34(-) | CD45(-) | CD11b/CD14(-) | CD19/CD79α(-) | HLA-DR(-)
其他常見標志物：CD44/CD29/CD200/CD166/CD146/CD271等

試劑盒技術創(chuàng)新

1. 標準化檢測方案

• 完整包含ISCT推薦的核心標志物組合

• 陽性混合液(FITC CD90 + PerCP-Cy™5.5 CD105 + APC CD73)

• 陰性混合液(PE CD45/CD34/CD11b/CD19/HLA-DR)

2. 模塊化設計優(yōu)勢

• 試劑盒內(nèi)附PE-CD44抗體

• 其他市售PE標記抗體

• 保留PE通道靈活性，可兼容：

• 支持用戶自定義擴展檢測項目

3. 實驗效率優(yōu)化

• 多色流式技術降低樣本需求量

• 預混抗體組合簡化操作流程

• 單獨陽性對照抗體便于補償調(diào)節(jié)

注：本方案適用于流式細胞術分析，建議配合同型對照設置和熒光補償對照使用，以獲得優(yōu)良檢測結果。

BD Stemflow 人 MSC 分析試劑盒 562245

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues.  MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes.  MSCs also have immunomodulatory effects in vivo and in vitro.  In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow.  Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR.  MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  In this kit we include the panel that was proposed by the ISCT.  In addition, we designed this kit to be modular so that additional markers could be "dropped-in".  Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates.  Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples.  Individual positive markers are included for compensation set-up.

準備和存儲

在 4°C 下原液保存，避免長時間光照。不要凍結。