產(chǎn)品介紹

BD GolgiPlug蛋白轉(zhuǎn)運(yùn)抑制劑(含BrefeldinA) 555029

BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A)

體外實(shí)驗中，向經(jīng)體外或體內(nèi)刺激的淋巴細(xì)胞添加BD GolgiPlug™（一種含布雷菲德菌素A的蛋白質(zhì)轉(zhuǎn)運(yùn)抑制劑）可阻斷其細(xì)胞內(nèi)蛋白質(zhì)轉(zhuǎn)運(yùn)過程。這導(dǎo)致細(xì)胞因子和/或蛋白質(zhì)在高爾基復(fù)合體中積累。細(xì)胞內(nèi)細(xì)胞因子聚集水平的增加，通過免疫熒光染色和流式細(xì)胞術(shù)分析，能夠增強(qiáng)對細(xì)胞因子分泌細(xì)胞的檢測靈敏度。

The ex vivo addition of BD GolgiPlug™, a protein transport inhibitor containing brefeldin A, to in vitro- or in vivo-stimulated lymphoid cells blocks their intracellular protein transport processes. This results in the accumulation of cytokines and/or proteins in the Golgi complex. This increased accumulation of cytokines in the cell enhances the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis.

BD GolgiPlug蛋白轉(zhuǎn)運(yùn)抑制劑(含BrefeldinA) 555029，BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A)

準(zhǔn)備和存儲

在 4°C 下未稀釋儲存。